Non-Invasive Mitochondrial DNA (MtDNA) BILOU Analysis Technique (Hylobates klossii, Miller 1903) Through Polymerase Chain Reaction

Abstract: Non-invasive analysis techniques for mitochondria bilou DNA (Hylobates klossii) with Polymerase Chain Reaction (PCR) are molecular techniques that can support animal welfare. Non-invasive analysis of stool samples can determine DNA samples from the control area in 12S rRNA. Fecal samples were collected from three different islands (Sipora, Siberut and Pagai) with a total of 39 samples extracted for DNA. Analysis was carried out at the LIPI Zoological Genetics laboratory in Cibinong in July to August 2005. The PCR test results used by Qiagen QIAamp ® DNA stool mini kit and electrophoresis can show that DNA samples can be displayed even in the lowest concentration of spectrophotometer measurements. Thus, this can be a new reference in future research as an indicator of conservation management in accordance with good animal welfare regulations. Polaroid photos from rRNA 12S (L = 1091, H = 1478) taken with UV transluminators and Polaroid MP4 cameras from PCR products with annealing were used at 50 ° C in 30 seconds, showing results from DNA electrophoresis marker bands ranging from 750 to 815 pb DNA ladder.

Abstract: Polymerase Chain Reaction is a non-invasive molecular technique that can support animal welfare. Non-invasive analysis from fecal sample can DNA samples from the control region on 12S rRNA. Fecal samples were collected from three different islands (Sipora, Siberut and Pagai) with a total of 39 samples were extracted for DNA. The analyzes were conducted by the Laboratory of Genetic Zoology LIPI, Cibinong in July until August 2005. The results of the PCR test used for Qiagen QIAamp® DNA stool kits and electrophoresis can show that the quality DNA sample displays from the fecal sample event in the lowest measurements of the spectrophotometer measurements. Thus, it has become a new reference in future research as an indicator of good management conservation in line with excellent animal welfare rules. The polaroid photos of the 12S rRNA (L = 1091, H = 1478) taken with UV translator and MP4 polaroid camera from the PCR products with annealing used at 50oC in 30 seconds, shows the results of the DNA sized marker band electrophoresis processes between 750 until 815 pb DNA ladder.

Key words: Hylobates klossii, bilou, mtDNA, PCR, 12S rRNA, non-invasive technique.