Nicotine Intervention on Low Density Lipoprotein Levels, and UCP-1 Expression (Uncoupling Protein 1) in Long-Tailed Monkeys (Macaca fascicularis) Obes at Risk of Atherogenesis

Abstract: Fifteen obese long tail monkeys (Macaca fascicularis) with the risk of atherogenesis were used to obtain information on levels of low density lipoprotein (LDL) and expression of uncoupling-1 protein (UCP-1) after three months of low-dose nicotine intervention orally. Animal groups were divided into three with different diet components, namely diet A (fat), type B diet (fat and egg yolk), and type C diet (commercial diet-monkey chow), and each group consisted of five monkeys. The three diets are mixed with nicotine and given to food a day at a dose of 0.50-0.75 mg / kg / 12 hours. Observation of blood lipids was carried out every month and at the time of necropsy brown adipose tissue was taken for evaluation by fluorescence antibody techniques. Based on lipid analysis showed that there was a significant increase in LDL concentration (p <0.05), especially for groups that received fat and egg feed. Based on the results of analysis of fluorescence antibody techniques, immunoreactive cells were obtained against UCP-1 expression during nicotine intervention.

Abstract: Fifteen obese cynomolgus monkeys (Macaca fascicularis) were used to obtain information on LDL level and to uncoupling protein-1 (UCP-1) expression during three months oral low dose nicotine intervention. Animals were grouped into three groups based on their diets, diet type A (tallow), type B (tall and egg yolk) and type C (commercial diet monkey chow), and each group consisted of five monkeys. Nicotine was mixed into diet with a dose of 0.50-0.75 mg / kg / 12 hours. Blood samples were collected every month for lipids and were taken for fluorescence antibody technique evaluation. Based on lipid analysis showed that there was a significant increase in LDL concentration (p <0.05), particularly for animals consuming diet type B. Fluorescent antibody evaluation was immunoreactive cells to UCP-1 expression during nicotine intervention.

Keyword: nicotine, expression UCP-1, Lipoprotein Low Density (LDL), cynomolgus