THE POTENCY OF CLOVE (SYZYGIUM AROMATICUM) ESSENTIAL OIL AS SLIMMING AROMATHERAPY BY IN VIVO ASSAY
Author : Fahmi Hasim, Irmanida Batubara, Irma Herawati Suparto
Abstract
Clove leaves (Syzygium aromaticum) contain essential oils which consists of eugenol, ß-caryophyllene and other compounds commonly used as perfume. The objective of this research was to analyze the potency of clove leave oil, eugenol, nonpolar extract, ß-caryophyllene and isoeugenol as slimming aromatherapy by in vivo assay. Clove oil was obtained by distillation and the oil was extracted using an alkaline solution until eugenol and nonpolar extract were obtained. ß-Caryophyllene was isolated from nonpolar extract using preparative thin layer chromatography. The crude essential oil and isolationproducted compounds were identified using gas chromatography-mass spectrometry. The results of in vivo assays showed that inhalation of isoeugenol had a potential for slimming by decreasing body fat tissues of rats. Lipid profile tests showed a decrease of triglyceride concentration in the clove oil group and the eugenol group compared to our negative control. In addition, there was a trend of decreasing body weight and total cholesterol concentration for the clove oil treatment.
Keywords: Clove oil, Essential oil, Isoeugenol, Slimming aromatherapy and Syzygium aromaticum
