Teknik Analisis Non-Invasif Mitokondria DNA (MtDNA) BILOU (Hylobates klossii, Miller 1903) Melalui Polymerase Chain Reaction
Abstract: Polymerase Chain Reaction is a non invasive molecular technique that can support animal welfare. Non-invasie analysis from fecal sample can determine DNA samples from the control region on 12S rRNA. Fecal samples were collected from three different islands (Sipora, Siberut and Pagai) with a total of 39 samples were extracted for DNA. The analyses were done at the laboratory of Genetic Zoology LIPI, Cibinong in July until August 2005. The results of the PCR test which used Qiagen QIAamp® DNA stool mini kit and electrophoresis can show that the sample displays the quality DNA from the fecal sample even in the lowest concentration of the spectrophotometer measurements. Thus, it has become a new reference in future research as an indicator of good management conservation in line with excellent animal welfare rules. The polaroid photos of the 12S rRNA (L=1091, H=1478) taken with UV transluminator and MP4 polaroid camera from the PCR products with annealing used at 50oC in 30 seconds, shows the results of this running process of electrophoresis DNA sized marker band processes between 750 until 815 pb DNA ladder.
Key words: Hylobates klossii, bilou, mtDNA, PCR, 12S rRNA, non-invasive technique.