Tuberculin Test on the Skin of Long Tailed Monkeys (Macaca fascicularis): In Prevention of Tuberculosis Zoonosis (TBC) in Weh Sabang Island Tourism Area

Erdiansyah Rahmi, Amalia Sutriana, Dwina Aliza

Abstract

Abstract: This study aims to obtain information about tuberculosis (TBC) transmission in long-tailed monkeys (Macaca fascicularis) in the tourist area of ​​Pulau Weh, Sabang and to support government programs in producing TB-free Indonesia. This research was conducted in several stages after obtaining approval and permission from the Aceh Center for Conservation and Natural Resources and related institutions in Sabang. The next stage determines the location, population, and identification of long-tailed monkeys and determines the number of long-tailed monkeys per group. Examination of animals by doing sedation using ketamine (10mg / kg) and xylazine (2 mg / kg) intramuscularly. Screening using the tuberculin test method (mammalian old tuberculin) was carried out on all animals 0.1 mL (135000 Tuberculin Units) intradermally on the palpebra. The tuberculin test results are read at 24, 48, and 72 hours after injection. The test was repeated in the second week. Data tabulated and analyzed descriptively. From the identification of monkey populations in the two regions one monkey group was found at zero kilometers (N 05 ° 54 ’19, 1 “E 95 ° 12 ‘57.4”) with a population of 14, while in the Cot Murong region (N 05 ° 54 ’19, 1 “E 95 ° 12 ‘57.4”) were found in two groups with a population of 12 and 14. Tuberculosis screening using this method showed that there was no incidence of tuberculosis in long-tailed monkeys in the tourist area of ​​Sabang.

Abstract: Infectious disease on long tail macaque (Macaca fascicularis) in Weh Island tourism area, Sabang and to support the government’s program in generating Indonesia free TB, as well. This research was conducted on many stages, they were permits with the BKSDA Aceh and Forestry Officer Sabang, location identification of the long tail macaque population, and identification of the macaque population in each group. The monkeys chosen were restrained and anesthetized using Ketamine (10mg / kg) and xylazine (2mg / kg) intramuscularly. The screening test using tuberculin skin test (TST) method (old mammalian tuberculin kit) was carried out to all samples, 0.1 mL (135000 tuberculin units) on palpebrae intradermally. The effect of TST was examined at 24, 48, and 72 hours post treatment. The test was repeated on the second week. The data were tabulated and analyzed descriptively. From the one group of macaca in Kilometer Zero (N 05 ° 54 ‘19,1 “E 95 ° 12’ 57,4”) with the population of 14 macacas, while in Cot Murong area ( N 05 ° 54 ‘19,1 “E 95 ° 12’ 57,4”) found two groups with the population of 12 and 14 macacas. The TB screening test using TST method showed that there were no incidents of TB in the long tail macaque in Sabang tourism area.

Keywords: Tuberculin Skin Test, Sabang, Macaca fascicularis