Novel Tetra- and Pentanucleotide Microsatellite Markers Allow For Multiplexed Genotyping of Sulawesi tarsiers (Tarsius spp.)
Author: Stefan Merker, Desiree Boucsein , Barbara Feldmeyer, Dyah Perwitasari-Farajallah, Bruno Streit
Abstract
Based on 454 sequencing, we characterized 10 polymorphic tetra- and pentanucleotide microsatellites for population studies of Sulawesi tarsiers. We identified 2516 repeat regions and designed PCR primer pairs for 60 tetra-, penta- and hexanucleotide repeat loci. Of 16 markers tested with dye-labelled primers, 11 proved to be polymorphic and 10 were readily amplified in multiplex PCR reactions. We tested 54 individuals of three species (T. lariang, T. dentatus, T. wallacei) and 10 putative hybrids for allelic diversity. All loci are genetically unlinked, one locus was found to deviate from Hardy–Weinberg equilibrium in one species. The novel marker system will aid studies of tarsier landscape genetics in the biodiversity hotspot Sulawesi.
Keywords : 454 Sequencing, Microsatellites, Primates, Sulawesi, Tarsius
